Human Claudin 18 ELISA Kit
Cat no : KE00766
Synonyms
Claudin18, Claudin-18, CLDN18, UNQ778/PRO1572
Validation Data Gallery
Product Information
KE00766 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human Claudin 18 ELISA kit is to be used to detect and quantify protein levels of endogenous human Claudin 18. The assay recognizes human Claudin 18. An antibody specific for human Claudin 18 has been pre-coated onto the microwells. The human Claudin 18 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human Claudin 18 is added to detect the captured human Claudin 18 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Claudin 18 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.01 ng/mL |
| Range | 0.188-12 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 51208 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 117% | 115%-121% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 7.78 | 0.38 | 4.84 |
| 2 | 8 | 1.52 | 0.03 | 2.18 |
| 3 | 8 | 0.81 | 0.02 | 2.37 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 7.15 | 0.78 | 10.91 |
| 2 | 16 | 1.40 | 0.13 | 9.26 |
| 3 | 16 | 0.77 | 0.05 | 6.43 |
Background Information
Claudin 18 is one of the most highly expressed claudin family members in lung alveolar epithelium, which are essential components of tight junctions in epithelial and endothelial cells. It is expressed at low levels in the airway epithelium and not in the lung endothelium. Claudin 18 specifically plays a role in alveolar fluid homeostasis by regulating the composition of alveolar epithelial tight junctions and ion transport. Additionally, soluble Claudin 18 levels may affect the pathogenesis of asthma.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Claudin18, Claudin-18, CLDN18, UNQ778/PRO1572 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.