Human Collagen Type I ELISA Kit
Cat no : KE00395
Synonyms
COL1, COL1A2, Alpha-2 type I collagen, col I, Collagen alpha 2(I) chain
Validation Data Gallery
Product Information
KE00395 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human Collagen Type I ELISA kit is to be used to detect and quantify protein levels of endogenous human Collagen Type I. The assay recognizes human Collagen Type I. An antibody specific for human Collagen Type I has been pre-coated onto the microwells. The human Collagen Type I protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human Collagen Type I is added to detect the captured human Collagen Type I protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Collagen Type I ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 0.09 ng/mL |
| Range | 0.313-20 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1278 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 90% | 86%-98% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 5.03 | 0.10 | 1.96 |
| 2 | 20 | 1.75 | 0.06 | 3.34 |
| 3 | 20 | 0.51 | 0.03 | 5.40 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 5.14 | 0.35 | 6.71 |
| 2 | 24 | 1.53 | 0.14 | 9.44 |
| 3 | 24 | 0.68 | 0.06 | 9.27 |
Background Information
Type I collagen is the main structural component of connective tissues such as skin, tendon and bone, and it is the most abundant and widely expressed collagen in human body. Collagen type I is a heterotrimer, which contains one α2(I) and two α1(I) chains, which are encoded by unlinked loci COL1A2 and COL1A1, respectively. COL1A2 gene mutation is associated with osteogenesis imperfecta, Ehlers-Danlos syndrome, idiopathic osteoporosis and atypical Marfan syndrome. This product mainly recognizes the C-terminal propeptide.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | COL1, COL1A2, Alpha-2 type I collagen, col I, Collagen alpha 2(I) chain |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
J Nanobiotechnology Platelet-hitchhike nanomedicine for theranostics of coronary artery dissection via X-CT imaging and inhibiting NLRP3 signaling pathway. | ||
NPJ Sci Food From rosemary and coffee to bioactive nanovesicles: exploring new frontiers in food functional ingredients. |