Human Complement C2 ELISA Kit
Cat no : KE00951
Synonyms
C2, C3/C5 convertase, CO2, Complement C2a fragment, Complement C2b fragment
Validation Data Gallery
Product Information
KE00951 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human Complement C2 ELISA kit is to be used to detect and quantify protein levels of endogenous human Complement C2. The assay recognizes human Complement C2. An antibody specific for human Complement C2 has been pre-coated onto the microwells. The human Complement C2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human Complement C2 is added to detect the captured human Complement C2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Complement C2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Saliva, Human milk |
| Assay type | Sandwich |
| Sensitivity | 0.01 ng/mL |
| Range | 0.2-12.5 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 717 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 73% | 71%-78% |
| Cell culture supernatant | 83% | 77%-86% |
| Saliva | 96% | 89%-104% |
| Human milk | 87% | 82%-89% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 5.40 | 0.15 | 2.76 |
| 2 | 8 | 1.63 | 0.06 | 3.92 |
| 3 | 8 | 0.78 | 0.04 | 5.65 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 5.54 | 0.37 | 6.68 |
| 2 | 16 | 1.58 | 0.08 | 5.12 |
| 3 | 16 | 0.75 | 0.05 | 6.55 |
Background Information
Complement C2 (C2) is a key component of the classical and lectin pathways of the complement system. Complement C2 deficiency is associated with an increased susceptibility to infections, particularly those caused by encapsulated bacteria.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | C2, C3/C5 convertase, CO2, Complement C2a fragment, Complement C2b fragment |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.