Human Complement factor H/CFH ELISA Kit
Cat no : KE00900
Synonyms
CFH, Complement factor H, ARMD4, ARMS1, Factor H
Validation Data Gallery
Product Information
KE00900 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human Complement factor H/CFH ELISA kit is to be used to detect and quantify protein levels of endogenous human Complement factor H/CFH. The assay recognizes human Complement factor H/CFH. An antibody specific for human Complement factor H/CFH has been pre-coated onto the microwells. The human Complement factor H/CFH protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human Complement factor H/CFH is added to detect the captured human Complement factor H/CFH protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Complement factor H/CFH ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Saliva |
| Assay type | Sandwich |
| Sensitivity | 10.8 pg/mL |
| Range | 46.88-3000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3075 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 108% | 103%-116% |
| Cell culture supernatant | 104% | 100%-106% |
| Saliva | 107% | 104%-108% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,480.2 | 27.7 | 1.9 |
| 2 | 8 | 325.7 | 24.0 | 7.4 |
| 3 | 8 | 200.3 | 7.5 | 3.8 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,421.4 | 74.1 | 5.2 |
| 2 | 16 | 335.1 | 31.8 | 9.5 |
| 3 | 16 | 192.7 | 11.9 | 6.2 |
Background Information
Complement factor H (CFH) is an abundant plasma glycoprotein that regulates the function of the alternative complement pathway in the fluid phase and on cellular surfaces. Factor H binds to C3b, accelerates the decay of the alternative pathway C3-convertase (C3bBb), and acts as a cofactor for the factor I-mediated proteolytic inactivation of C3b. It plays a critical role in the homeostasis of the complement system in plasma and in the protection of bystander host cells and tissues from damage by complement activation. Abnormalities in factor H have been associated with renal disease.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CFH, Complement factor H, ARMD4, ARMS1, Factor H |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Front Immunol Small extracellular vesicles proteome reveals persistent inflammatory and coagulopathic dysregulation in long-COVID. |