Human Cytokeratin 7 ELISA Kit
Cat no : KE00709
Synonyms
KRT7, CK 7, CK7, CK-7, Cytokeratin,Keratin
Validation Data Gallery
Product Information
KE00709 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human Cytokeratin 7 ELISA kit is to be used to detect and quantify protein levels of endogenous human Cytokeratin 7. The assay recognizes human Cytokeratin 7. An antibody specific for human Cytokeratin 7 has been pre-coated onto the microwells. The human Cytokeratin 7 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human Cytokeratin 7 is added to detect the captured human Cytokeratin 7 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Cytokeratin 7 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 1.04 ng/mL |
| Range | 2.5-160 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3855 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 93% | 83%-110% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 80.82 | 5.23 | 6.48 |
| 2 | 8 | 20.22 | 1.73 | 8.57 |
| 3 | 8 | 11.21 | 1.16 | 10.37 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 79.53 | 5.73 | 7.20 |
| 2 | 16 | 20.58 | 1.74 | 8.44 |
| 3 | 16 | 11.71 | 1.13 | 9.61 |
Background Information
Cytokeratin 7 (CK7) is a type of low molecular weight cytokeratin that is generally restricted to epithelia and their neoplasms. It is commonly expressed in glandular epithelial cells, such as those in the lung, breast, and endometrium. CK7 is often used as a diagnostic marker in pathology to differentiate between various types of carcinomas. For instance, CK7 is typically positive in adenocarcinomas of the lung, breast, and endometrium, while it is usually negative in colorectal adenocarcinomas. Additionally, CK7 has been implicated in tumor progression and metastasis. Its overexpression has been associated with poor prognosis in certain cancers, such as pancreatic adenocarcinoma. CK7 is often paired with CK20 in diagnostic pathology to classify tumors.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | KRT7, CK 7, CK7, CK-7, Cytokeratin,Keratin |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.