Human DCBLD2 ELISA Kit
Cat no : KE00816
Synonyms
CLCP1, CUB, LCCL and coagulation factor V/VIII-homology domains protein 1, Discoidin, CUB and LCCL domain-containing protein 2, Endothelial and smooth muscle cell-derived neuropilin-like protein, ESDN
Validation Data Gallery
Product Information
KE00816 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human DCBLD2 ELISA kit is to be used to detect and quantify protein levels of endogenous human DCBLD2. The assay recognizes human DCBLD2. An antibody specific for human DCBLD2 has been pre-coated onto the microwells. The human DCBLD2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human DCBLD2 is added to detect the captured human DCBLD2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human DCBLD2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.8 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 131566 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 123% | 112%-132% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,837.6 | 95.6 | 5.2 |
| 2 | 8 | 438.2 | 20.1 | 4.6 |
| 3 | 8 | 211.8 | 6.7 | 3.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,764.5 | 161.9 | 9.2 |
| 2 | 16 | 415.8 | 32.4 | 7.8 |
| 3 | 16 | 203.0 | 11.9 | 5.9 |
Background Information
DCBLD2 (also known as CLCP1 or ESDN) is a transmembrane protein belonging to the DCBLD family, characterized by extracellular discoidin, CUB, and LCCL domains. It plays roles in cell adhesion, signaling, and vascular biology, with emerging implications in cancer progression, angiogenesis, and cardiovascular diseases. DCBLD2 is upregulated in certain tumors (e.g., glioblastoma, lung cancer) and may regulate receptor tyrosine kinase (RTK) pathways, including EGFR and PDGFR. Its exact mechanisms remain under investigation, but it is suggested to influence cell migration, proliferation, and tumor microenvironment interactions.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CLCP1, CUB, LCCL and coagulation factor V/VIII-homology domains protein 1, Discoidin, CUB and LCCL domain-containing protein 2, Endothelial and smooth muscle cell-derived neuropilin-like protein, ESDN |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.