Human DLL3 ELISA Kit
Cat no : KE00627
Synonyms
delta like 3 (Drosophila), Delta like protein 3, Delta3, Delta-like protein 3, Drosophila Delta homolog 3
Validation Data Gallery
Product Information
KE00627 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human DLL3 ELISA kit is to be used to detect and quantify protein levels of endogenous human DLL3. The assay recognizes human DLL3. An antibody specific for human DLL3 has been pre-coated onto the microwells. The human DLL3 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human DLL3 is added to detect the captured human DLL3 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human DLL3 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.22 ng/mL |
| Range | 0.25-16 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 10683 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 92% | 88%-95% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 3.68 | 0.25 | 6.85 |
| 2 | 8 | 0.96 | 0.08 | 7.95 |
| 3 | 8 | 0.50 | 0.03 | 6.04 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 3.59 | 0.27 | 7.39 |
| 2 | 16 | 0.90 | 0.10 | 10.57 |
| 3 | 16 | 0.50 | 0.05 | 9.53 |
Background Information
The Delta-Notch pathway is an evolutionarily conserved signaling pathway which controls a broad range of developmental processes including cell fate determination, terminal differentiation and proliferation. In mammals, four Notch receptors (NOTCH1-4) and five activating canonical ligands (JAGGED1, JAGGED2, DLL1, DLL3 and DLL4) have been described. DLL3 is an inhibitory ligand of the Notch signaling pathway that is predominantly localizes to the Golgi apparatus in normal condition. Normal tissue expression of DLL3 is highest in fetal brain, and DLL3 plays a key role in somitogenesis in the paraxial mesoderm. It has been reported that DLL3 is expressed on the surface of tumor cells of small cell lung cancer (SCLC) and high-grade neuroendocrine carcinomas (LCNEC) and has emerged as a novel therapeutic target.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | delta like 3 (Drosophila), Delta like protein 3, Delta3, Delta-like protein 3, Drosophila Delta homolog 3 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.