Human DNMT3A ELISA Kit
Cat no : KE00812
Synonyms
Cysteine methyltransferase DNMT3A, DNA (cytosine-5)-methyltransferase 3A, DNA MTase HsaIIIA, EC:2.1.1.-, EC:2.1.1.37
Validation Data Gallery
Product Information
KE00812 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human DNMT3A ELISA kit is to be used to detect and quantify protein levels of endogenous human DNMT3A. The assay recognizes human DNMT3A. An antibody specific for human DNMT3A has been pre-coated onto the microwells. The human DNMT3A protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human DNMT3A is added to detect the captured human DNMT3A protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human DNMT3A ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.06 ng/mL |
| Range | 0.25-16 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1788 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 111% | 99%-122% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 9.39 | 0.76 | 8.05 |
| 2 | 8 | 2.02 | 0.05 | 2.53 |
| 3 | 8 | 1.07 | 0.04 | 3.96 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 9.29 | 0.87 | 9.34 |
| 2 | 16 | 1.92 | 0.20 | 10.42 |
| 3 | 16 | 1.06 | 0.04 | 3.94 |
Background Information
DNMT3A (DNA Methyltransferase 3A) is a key enzyme involved in de novo DNA methylation, adding methyl groups to cytosine residues in CpG dinucleotides. It plays crucial roles in embryonic development, genetic imprinting, and the regulation of gene expression. DNMT3A is essential for establishing initial methylation patterns and is highly expressed in embryonic stem cells. Mutations or dysregulation of DNMT3A have been implicated in various cancers and developmental disorders. Its interaction with other proteins, such as DNMT3L, further modulates its activity and function.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Cysteine methyltransferase DNMT3A, DNA (cytosine-5)-methyltransferase 3A, DNA MTase HsaIIIA, EC:2.1.1.-, EC:2.1.1.37 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Pharmaceuticals (Basel) From Food Contaminant to Therapeutic Target: Identification of KCNE2 and 5-Azacytidine for Gastric Cancer via Multi-Omics, Machine Learning, and In Vitro Validation. |