Human DPP4/CD26 ELISA Kit
Cat no : KE00292
Synonyms
CD26, ADABP, ADCP 2, ADCP2, ADCP-2
Validation Data Gallery
Product Information
KE00292 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human DPP4/CD26 ELISA kit is to be used to detect and quantify protein levels of endogenous human DPP4/CD26. The assay recognizes human DPP4/CD26. An antibody specific for human DPP4/CD26 has been pre-coated onto the microwells. The human DPP4/CD26 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human DPP4/CD26 is added to detect the captured human DPP4/CD26 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human DPP4/CD26 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Urine, Saliva |
| Assay type | Sandwich |
| Sensitivity | 0.03 ng/mL |
| Range | 0.313-20 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1803 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Serum | 87% | 71%-102% |
| Cell culture supernatant | 87% | 81%-94% |
| Urine | 89% | 78%-94% |
| Saliva | 91% | 81%-103% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 9.78 | 0.23 | 2.39 |
| 2 | 20 | 2.43 | 0.03 | 1.32 |
| 3 | 20 | 0.62 | 0.02 | 2.53 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 9.70 | 0.23 | 2.41 |
| 2 | 24 | 2.42 | 0.07 | 2.78 |
| 3 | 24 | 0.59 | 0.02 | 2.99 |
Background Information
Dipeptidyl peptidase 4 (DPP4, also known as CD26) is a serine exopeptidase that cleaves X-proline dipeptides from the N terminus of polypeptides. It is a type II membrane protein consisting of a short cytoplasmic tail, a transmembrane domain, and a large extracellular domain comprised of a flexible stalk, glycosylation-rich region, cysteine-rich region and catalytic region. DPP4 is widely expressed on T cells, activated B cells, activated NK cells and myeloid cells as well as on epithelial, endothelial cells of a variety of tissues. A soluble form of DPP4 lacking the intracellular part and the transmembrane region is found in body fluids. Altered levels of serum soluble DPP4 have been reported in many diseases including inflammatory and infectious diseases, and tumors.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD26, ADABP, ADCP 2, ADCP2, ADCP-2 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Int J Obes (Lond) miR-548ag promotes DPP4 expression in hepatocytes through activation of TLR(7/8)/NF-κB pathway |