Human DR5 ELISA Kit
Cat no : KE00731
Synonyms
CD262, Death receptor 5, KILLER, KILLER/DR5, TNF-related apoptosis-inducing ligand receptor 2
Validation Data Gallery
Product Information
KE00731 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human DR5 ELISA kit is to be used to detect and quantify protein levels of endogenous human DR5. The assay recognizes human DR5. An antibody specific for human DR5 has been pre-coated onto the microwells. The human DR5 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human DR5 is added to detect the captured human DR5 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human DR5 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 3.0 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 8795 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 89% | 88%-90% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 470.0 | 49.3 | 10.5 |
| 2 | 8 | 106.9 | 3.9 | 3.6 |
| 3 | 20 | 64.6 | 4.4 | 6.8 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 476.1 | 54.1 | 11.4 |
| 2 | 16 | 107.7 | 5.4 | 5.0 |
| 3 | 16 | 61.9 | 5.0 | 8.0 |
Background Information
Death receptor 5 (DR5) is a member of the tumor necrosis factor receptor superfamily. It plays a critical role in regulating apoptosis through its interaction with the TNF-related apoptosis-inducing ligand (TRAIL). When DR5 is activated by TRAIL, it triggers a cascade of intracellular signaling events that lead to the activation of caspases, the proteases responsible for executing apoptosis. This selective targeting of cancer cells by DR5 has made it a promising candidate for cancer therapy. Elevated levels of soluble DR5 have been observed in the serum of cancer patients, indicating its potential as a biomarker.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD262, Death receptor 5, KILLER, KILLER/DR5, TNF-related apoptosis-inducing ligand receptor 2 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.