Human DSG1 ELISA Kit
Cat no : KE00705
Synonyms
Cadherin family member 4, CDHF4, desmoglein 1, Desmoglein-1, Desmosomal glycoprotein 1
Validation Data Gallery
Product Information
KE00705 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human DSG1 ELISA kit is to be used to detect and quantify protein levels of endogenous human DSG1. The assay recognizes human DSG1. An antibody specific for human DSG1 has been pre-coated onto the microwells. The human DSG1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human DSG1 is added to detect the captured human DSG1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human DSG1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 11.7 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1828 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 95% | 85%-108% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,234.5 | 149.6 | 6.7 |
| 2 | 8 | 570.4 | 38.4 | 6.7 |
| 3 | 8 | 301.1 | 16.6 | 5.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,237.3 | 176.6 | 7.9 |
| 2 | 16 | 575.6 | 38.8 | 6.7 |
| 3 | 16 | 287.8 | 26.2 | 9.1 |
Background Information
Desmoglein-1 (DSG1) is a member of the desmoglein subfamily of proteins, which are key components of desmosomes, specialized cell-cell junctions that provide mechanical strength to tissues. DSG1 is crucial for maintaining the integrity of the epidermis and other epithelial tissues. Mutations in the DSG1 gene have been linked to various skin disorders, including pemphigus foliaceus, an autoimmune blistering disease, and palmoplantar keratoderma. Additionally, DSG1 expression is often downregulated in certain cancers, such as melanoma, where its loss can promote tumor cell migration. Recent studies have also shown that DSG1 expression can be affected by other genetic factors, such as PAX6 knockdown in human limbal epithelial cells. DSG1 is also involved in paracrine signaling, influencing the behavior of melanocytes.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Cadherin family member 4, CDHF4, desmoglein 1, Desmoglein-1, Desmosomal glycoprotein 1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.