Human/Mouse Dystrophin ELISA Kit
Cat no : KE00479
Synonyms
DMD,BMD,Dystrophin
Validation Data Gallery
Product Information
KE00479 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human Dystrophin ELISA kit is to be used to detect and quantify protein levels of endogenous human Dystrophin. The assay recognizes human Dystrophin. An antibody specific for human Dystrophin has been pre-coated onto the microwells. The human Dystrophin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human Dystrophin is added to detect the captured human Dystrophin protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human/Mouse Dystrophin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate, Tissue lysate, Tissue homogenate |
| Assay type | Sandwich |
| Sensitivity | 4.8 pg/mL |
| Range | 39.1-2500 pg/mL |
| Reactivity | Human/Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1756 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 88% | 83%-95% |
| Tissue lysate | 79% | 77%-81% |
| Tissue homogenates | 92% | 87%-97% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,020.4 | 35.0 | 3.4 |
| 2 | 8 | 303.7 | 11.5 | 3.8 |
| 3 | 8 | 142.4 | 3.2 | 2.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,090.0 | 76.7 | 7.0 |
| 2 | 16 | 291.2 | 16.6 | 5.7 |
| 3 | 16 | 147.4 | 5.9 | 4.0 |
Background Information
Dystrophin (DMD or BMD) is a large muscle protein whose mutations cause Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD), the childhood neuromuscular disorders that result in progressive muscle weakness, respiratory difficulties and cardiovascular dysfunction. Dystrophin is a crucial component of the dystrophin-glycoprotein complex which is essential for muscle membrane integrity and stability. Dystrophin is located on the cytoplasmic face of the sarcolemma and connects the cytoskeletal network to the sarcolemma and extracellular matrix.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | DMD,BMD,Dystrophin |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.