Human E-cadherin ELISA Kit
Cat no : KE00818
Synonyms
Arc 1, Cadherin 1, Cadherin-1, CAM 120/80, CD324
Validation Data Gallery
Product Information
KE00818 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human E-cadherin ELISA kit is to be used to detect and quantify protein levels of endogenous human E-cadherin. The assay recognizes human E-cadherin. An antibody specific for human E-cadherin has been pre-coated onto the microwells. The human E-cadherin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human E-cadherin is added to detect the captured human E-cadherin protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human E-cadherin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Urine, Saliva |
| Assay type | Sandwich |
| Sensitivity | 0.17 ng/mL |
| Range | 0.47-30 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 999 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 97% | 87%-106% |
| Urine | 105% | 101%-110% |
| Saliva | 79% | 73%-84% |
| Cell culture supernatant | 99% | 97%-103% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 14.87 | 0.49 | 3.30 |
| 2 | 8 | 3.91 | 0.12 | 3.00 |
| 3 | 8 | 2.20 | 0.08 | 3.77 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 14.02 | 1.11 | 7.90 |
| 2 | 16 | 3.69 | 0.30 | 8.03 |
| 3 | 16 | 2.10 | 0.13 | 6.33 |
Background Information
E-cadherin is a transmembrane glycoprotein that connects epithelial cells at adherens junctions. In normal cells, E-cadherin exerts its tumor-suppressing role mainly by sequestering β-catenin from its binding to LEF (Lymphoid enhancer factor)/TCF (T cell factor), which serves the function of transcribing genes of the proliferative Wnt signaling pathway. The loss of E-cadherin expression associated with the epithelial-mesenchymal transition (EMT) occurs frequently during tumor metastasis. Furthermore, Soluble E-cadherin (an 80-kDa soluble form) may be a biomarker for the diagnosis and prognosis of cancer.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Arc 1, Cadherin 1, Cadherin-1, CAM 120/80, CD324 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.