Human E-selectin / CD62E ELISA Kit
Cat no : KE00611
Synonyms
CD62E, E-selectin, CD62 antigen-like family member E, E selectin, ELAM
Validation Data Gallery
Product Information
KE00611 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human E-selectin / CD62E ELISA kit is to be used to detect and quantify protein levels of endogenous human E-selectin / CD62E. The assay recognizes human E-selectin / CD62E. An antibody specific for human E-selectin / CD62E has been pre-coated onto the microwells. The human E-selectin / CD62E protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human E-selectin / CD62E is added to detect the captured human E-selectin / CD62E protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human E-selectin / CD62E ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 2.8 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6401 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 106% | 103%-112% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 447.8 | 19.4 | 4.3 |
| 2 | 8 | 119.4 | 1.6 | 1.4 |
| 3 | 8 | 60.8 | 3.8 | 6.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 452.7 | 16.1 | 3.6 |
| 2 | 16 | 116.5 | 4.1 | 3.5 |
| 3 | 16 | 59.1 | 5.1 | 8.6 |
Background Information
E-selectin (Endothelial Leukocyte Adhesion Molecule-1, also known as CD62E) is a critical adhesion molecule expressed on endothelial cells. It plays a crucial role in the initial steps of leukocyte recruitment to sites of inflammation, facilitating the rolling and adhesion of leukocytes to the endothelium. This process is essential for the immune response to infection and injury. E-selectin is upregulated in response to inflammatory cytokines such as IL-1, TNF-α, and bacterial lipopolysaccharide (LPS), and it interacts with various glycan ligands on leukocytes, including PSGL-1, ESL-1, CD44, and CD43.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD62E, E-selectin, CD62 antigen-like family member E, E selectin, ELAM |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.