Human ECM1 ELISA Kit

Sensitivity

0.1 pg/mL

Range

78.1-5000 pg/mL

Reactivity

Human

Cat no : KE00141

Synonyms

ECM1, extracellular matrix protein 1, Secretory component p85



Product Information

KE00141 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The ECM1 ELISA kit is to be used to detect and quantify protein levels of endogenous ECM1. The assay recognizes human ECM1. An antibody specific for ECM1 has been pre-coated onto the microwells. The ECM1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for ECM1 is added to detect the captured ECM1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm .

Product name Human ECM1 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 0.1 pg/mL
Range 78.1-5000 pg/mL
Reactivity Human
Tested applications Sandwich ELISA
Gene ID (NCBI) 1893

Recovery

Sample TypeAverageRange
Human serum99%81%-114%
Cell culture supernatants85%71%-96%

IntraAssay

Samplenmean ( pg/mL)SDCV%
1202,305.546.32.0
220615.724.94.1
320116.99.48.0

InterAssay

Samplenmean ( pg/mL)SDCV%
1242,070.5108.75.3
224572.829.95.2
324152.815.910.4

Background Information

Extracellular matrix protein 1 (ECM1) is a glycoprotein involved in a number of biological processes such as bone formation, skin differentiation, cell proliferation, and promotes angiostasis. ECM1 is expressed in breast cancer and could participate in cell migration and angiogenesis. Pathologically, ECM1 contributes to the formation and metastasis of several types of cancer including breast, thyroid and hepatocellular cancers. It can be detected in human serum. ECM1 protein is a possible trigger for angiogenesis, tumor progression and malignancies.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms ECM1, extracellular matrix protein 1, Secretory component p85