Speedy™ Human ECM1 One-Step ELISA Kit
Cat no : SE50051
Synonyms
extracellular matrix protein 1, Secretory component p85
Validation Data Gallery
Product Information
| Product name | Speedy™ Human ECM1 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 2.8 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1893 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 91% | 82%-101% |
| Cell culture supernatant | 109% | 98%-123% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 509.8 | 7.8 | 1.5 |
| 2 | 8 | 123.0 | 1.4 | 1.1 |
| 3 | 8 | 37.2 | 0.5 | 1.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 516.2 | 14.8 | 2.9 |
| 2 | 16 | 125.1 | 2.6 | 2.1 |
| 3 | 16 | 37.5 | 0.7 | 1.9 |
Background Information
Extracellular matrix protein 1 (ECM1) is a glycoprotein involved in a number of biological processes such as bone formation, skin differentiation, cell proliferation, and promotes angiostasis. ECM1 is expressed in breast cancer and could participate in cell migration and angiogenesis. Pathologically, ECM1 contributes to the formation and metastasis of several types of cancer including breast, thyroid and hepatocellular cancers. It can be detected in human serum. ECM1 protein is a possible trigger for angiogenesis, tumor progression and malignancies.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | extracellular matrix protein 1, Secretory component p85 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.