Human NSE/ENO2 ELISA Kit
Cat no : KE00050
Synonyms
ENO2, NSE, 2-phospho-D-glycerate hydro-lyase, EC:4.2.1.11, Enolase 2
Validation Data Gallery
Product Information
KE00050 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human NSE/ENO2 ELISA kit is to be used to detect and quantify protein levels of endogenous human NSE/ENO2. The assay recognizes human NSE/ENO2. An antibody specific for human NSE/ENO2 has been pre-coated onto the microwells. The human NSE/ENO2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human NSE/ENO2 is added to detect the captured human NSE/ENO2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human NSE/ENO2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 0.11 ng/mL |
| Range | 0.313-20 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2026 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 87% | 71%-118% |
| Cell culture supernatants | 90% | 70%-110% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 2.3 | 0.1 | 6.0 |
| 2 | 20 | 7.6 | 0.6 | 7.8 |
| 3 | 20 | 16.1 | 0.9 | 5.5 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 2.3 | 0.1 | 5.7 |
| 2 | 24 | 7.4 | 0.4 | 5.6 |
| 3 | 24 | 12.9 | 0.8 | 5.8 |
Background Information
ENO2, also named as Enolases are cytoplasmic glycolytic enzymes that may be involved in differentiation. The enolase has three isoenzymes, alpha, beta and gamma. The alpha form is expressed in most tissues, whereas the beta form is expressed in muscle tissue. The gamma enolase (ENO2), particularly isoform neuron-specific enolase (NSE), a homodimer, is primarily localized in neurons and neuroendocrine cells and is a cancer diagnostic marker for brain tumors. ENO2 plays a role in the glycolysis-related energy pathway and might be involved in higher metabolic activity during the day than at night, at least in part.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ENO2, NSE, 2-phospho-D-glycerate hydro-lyase, EC:4.2.1.11, Enolase 2 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Nat Commun A tissue-bioengineering strategy for modeling rare human kidney diseases in vivo. | ||
Medicine (Baltimore) Plasma S100β and neuron-specific enolase, but not neuroglobin, are associated with early cognitive dysfunction after total arch replacement surgery: A pilot study. | ||
J Mol Histol Gamma-enolase predicts lung damage in severe acute pancreatitis-induced acute lung injury. | ||
Drug Alcohol Depend Peripheral inflammation and PSD-95 levels are associated with structural brain changes in individuals with nicotine dependence using e-cigarettes. | ||
Front Aging Neurosci Transcutaneous electrical acupoint stimulation for reducing cognitive dysfunction in lumbar spine surgery: A randomized, controlled trail | ||
Ann Surg MiR-7-5p as a Diagnostic and Monitoring Biomarker for Neuroendocrine Tumors: Insights from the SKY-NET Multicenter Study |