Human ENPP-2/Autotaxin ELISA Kit
Cat no : KE00179
Synonyms
ATX, ATX X, AUTOTAXIN, E NPP 2, ENPP2, FLJ26803, LysoPLD, PD IALPHA, PDNP2
Validation Data Gallery
Product Information
KE00179 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The ENPP-2/Autotaxin ELISA kit is to be used to detect and quantify protein levels of endogenous ENPP-2/Autotaxin. The assay recognizes human ENPP-2/Autotaxin. An antibody specific for ENPP-2/Autotaxin has been pre-coated onto the microwells. The ENPP-2/Autotaxin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human ENPP-2/Autotaxin is added to detect the captured human ENPP-2/Autotaxin protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human ENPP-2/Autotaxin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 0.01 ng/mL |
| Range | 0.625-40 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 5168 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Serum | 93% | 70%-110% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 2.53 | 0.09 | 3.5 |
| 2 | 20 | 5.95 | 0.19 | 3.1 |
| 3 | 20 | 19.19 | 0.30 | 1.6 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 2.35 | 0.11 | 4.6 |
| 2 | 24 | 5.66 | 0.17 | 3.0 |
| 3 | 24 | 18.53 | 0.48 | 2.6 |
Background Information
ENPP2, Autotaxin or Extracellular lysophospholipase D, is a 863 amino acid secreted protein, which is detected in blood plasma (at protein level) (PubMed:12176993, PubMed:26371182). ENPP2 is Predominantly expressed in brain, placenta, ovary, and small intestine. ENPP2 is expressed in a number of carcinomas such as hepatocellular and prostate carcinoma, neuroblastoma and non-small-cell lung cancer. ENPP2 is expressed in body fluids such as plasma, cerebral spinal fluid (CSF), saliva, follicular and amniotic fluids. Biliary atresia patients had greater serum autotaxin and liver stiffness than controls. Serum autotaxin and liver stiffness were markedly elevated in Biliary atresia patients with jaundice compared to those without jaundice. Furthermore, serum autotaxin was correlated with liver stiffness and biochemical parameters in Biliary atresia. This kit is used to quality ENPP2 level in serum.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ATX, ATX X, AUTOTAXIN, E NPP 2, ENPP2, FLJ26803, LysoPLD, PD IALPHA, PDNP2 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Pediatr Pulmonol Association between plasma lysophosphatidic acid levels and bronchopulmonary dysplasia in extremely preterm infants: A prospective study | ||
Anal Chim Acta Establishment and clinical application evaluations of a deep mining strategy of plasma proteomics based on nanomaterial protein coronas |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH CLAIRE (Verified Customer) (08-06-2024) | Easy to use, clear instructions
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