Speedy™ Human EPO/Erythropoietin One-Step ELISA Kit
Cat no : SE50274
Synonyms
EPO, EP, Epoetin, erythropoietin, MVCD2
Validation Data Gallery
Product Information
| Product name | Speedy™ Human EPO/Erythropoietin One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 0.2 mIU/mL |
| Range | 4.7-300 mIU/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2056 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 104% | 84%-129% |
IntraAssay
| Sample | n | mean ( mIU/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 155.9 | 2.7 | 1.7 |
| 2 | 8 | 38.4 | 1.0 | 2.5 |
| 3 | 8 | 20.7 | 0.5 | 2.5 |
InterAssay
| Sample | n | mean ( mIU/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 158.1 | 3.1 | 2.0 |
| 2 | 16 | 38.6 | 0.9 | 2.3 |
| 3 | 16 | 20.5 | 0.5 | 2.6 |
Background Information
Erythropoietin (EPO) is a glycoprotein hormone that regulates the production of red blood cells and biosynthesis of hemoglobin. The predominant expression of this gene shifts from the liver during fetal development to kidney in adults, and and the secreted protein will travel through the blood stream to reach to the bone marrow to stimulate hematopoietic stem cell differentiation to RBC. EPO binds to the cognate EPO receptor (EPOR) on erythroid progenitor cells, thus preventing apoptosis and stimulating their differentiation and maturation into erythrocyte. However, EPO protein and its receptors have also been shown to be cytoprotective in extra-hematopoietic tissues including the retina tissue. Low levels of EPO (around 10 mIU/mL) are constantly secreted sufficient to compensate for normal red blood cell turnover. Common causes of cellular hypoxia resulting in elevated levels of EPO (up to 10000 mIU/mL) include any anemia, and hypoxemia due to chronic lung disease.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | EPO, EP, Epoetin, erythropoietin, MVCD2 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.