Human ETV4 ELISA Kit
Cat no : KE00518
Synonyms
ETV4,Adenovirus E1A enhancer-binding protein,E1AF,
Validation Data Gallery
Product Information
KE00518 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human ETV4 ELISA kit is to be used to detect and quantify protein levels of endogenous human ETV4. The assay recognizes human ETV4. An antibody specific for human ETV4 has been pre-coated onto the microwells. The human ETV4 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human ETV4 is added to detect the captured human ETV4 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human ETV4 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.02 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2118 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 84% | 80%-87% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 5.26 | 0.21 | 4.04 |
| 2 | 8 | 1.28 | 0.05 | 4.21 |
| 3 | 8 | 0.66 | 0.04 | 5.48 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 5.01 | 0.39 | 7.79 |
| 2 | 16 | 1.25 | 0.06 | 4.96 |
| 3 | 16 | 0.66 | 0.04 | 6.49 |
Background Information
ETS translocation variant 4 (ETV4), also named as E1A-F or PEA3, is 484 amino acid protein, which contains one ETS DNA-binding domain and belongs to the ETS family. ETV4 as a transcription activator localizes in the nucleus and binds to the enhancer of the adenovirus E1A gene. ETV4 is a key transcription factor in regulating glycolytic gene expression and its expression is positively correlated with glycolytic signaling.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ETV4,Adenovirus E1A enhancer-binding protein,E1AF, |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.