Human ErbB3/HER3 ELISA Kit
Cat no : KE00760
Synonyms
ERBB3, c erbB 3, c erbB3, EC:2.7.10.1, ErbB 3
Validation Data Gallery
Product Information
KE00760 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human ErbB3/HER3 ELISA kit is to be used to detect and quantify protein levels of endogenous human ErbB3/HER3. The assay recognizes human ErbB3/HER3. An antibody specific for human ErbB3/HER3 has been pre-coated onto the microwells. The human ErbB3/HER3 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human ErbB3/HER3 is added to detect the captured human ErbB3/HER3 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human ErbB3/HER3 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Urine, Saliva, Human milk, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 3.4 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2065 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 87% | 76%-98% |
| Cell culture supernatant | 105% | 101%-111% |
| Urine | 98% | 94%-100% |
| Saliva | 100% | 99%-103% |
| Human milk | 94% | 88%-97% |
| Cell lysate | 96% | 92%-102% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 811.9 | 7.7 | 1.0 |
| 2 | 8 | 201.5 | 5.4 | 2.7 |
| 3 | 8 | 103.3 | 6.6 | 6.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 796.8 | 19.6 | 2.5 |
| 2 | 16 | 201.5 | 5.0 | 2.5 |
| 3 | 16 | 105.2 | 5.8 | 5.5 |
Background Information
ErbB3/HER3 is a member of the ErbB family of receptor tyrosine kinases, which play crucial roles in cell proliferation, survival, and differentiation. ErbB3/HER3 is a type I transmembrane glycoprotein and is unique within the ErbB family because it lacks intrinsic kinase activity. Instead, it relies on forming heterodimers with other ErbB receptors, particularly HER2, to mediate signaling. HER3 has emerged as a key player in resistance to other HER family-targeted therapies, such as tyrosine kinase inhibitors and monoclonal antibodies against EGFR or HER2.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ERBB3, c erbB 3, c erbB3, EC:2.7.10.1, ErbB 3 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Free Radic Biol Med Activation of the Lp-PLA2/LPC axis triggers endothelial ferroptosis to drive diabetic kidney disease. |