β-Estradiol ELISA Kit
Cat no : KE30010
Synonyms
17β-Estradiol
Validation Data Gallery
Product Information
KE30010 is a vitro competitive binding enzyme-linked immunosorbent assay(Competitive ELISA). The β-Estradiol ELISA kit is to be used to detect and quantify protein levels of vitro human β-Estradiol. The assay recognizes human β-Estradiol. A 96-well plate has been precoated with β-Estradiol antigen. Samples and the antibody-Bio conjugate are added to the wells, where β-Estradiol in the sample competes with the β-Estradiol antigen immobilized on the microplate for binding with the antibody-Bio. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of β-Estradioll in the sample and the intensity is measured at 450 nm with the correction wavelength set at 630 nm.
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 97% | 84%-128% |
| Urine | 99% | 93%-124% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,550.9 | 68.9 | 4.4 |
| 2 | 8 | 394.1 | 34.4 | 8.7 |
| 3 | 8 | 191.2 | 16.0 | 8.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,539.4 | 72.7 | 4.7 |
| 2 | 16 | 391.1 | 36.5 | 9.3 |
| 3 | 16 | 176.6 | 24.9 | 14.1 |
Background Information
β-Estradiol is the primary and most potent estrogen sex hormone in the human body, particularly in non-pregnant females of reproductive age. It is a steroid hormone essential for the development and regulation of the female reproductive system, secondary sexual characteristics, and numerous other physiological processes.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | 17β-Estradiol |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.