Human FGF2 ELISA Kit
Cat no : KE00129
Synonyms
Basic fibroblast growth factor, BFGF, FGF2, FGFB, HBGF 2
Validation Data Gallery
Product Information
KE00129 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The FGF2 ELISA kit is to be used to detect and quantify protein levels of endogenous FGF2. The assay recognizes human FGF2. An antibody specific for FGF2 has been pre-coated onto the microwells. The FGF2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for FGF2 is added to detect the captured FGF2 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human FGF2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysates |
| Assay type | Sandwich |
| Sensitivity | 18.8 pg/mL |
| Range | 31.25-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2247 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysates | 95% | 81%-124% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 507.6 | 25.6 | 5.0 |
| 2 | 20 | 110.7 | 6.3 | 5.7 |
| 3 | 20 | 62.3 | 3.0 | 4.8 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 500.7 | 21.9 | 4.4 |
| 2 | 24 | 114.3 | 9.8 | 8.6 |
| 3 | 24 | 63.3 | 3.1 | 4.9 |
Background Information
Fibroblast growth factor 2 (FGF2) is one of the most well-studied members of the fibroblast growth factor superfamil. FGF2 can be synthesized and secreted by human adipocytes. FGF2 activates its target receptor tyrosine kinases, the FGFRs, on the cell surface in order to activate numerous downstream pathways, including several mitogen activated protein kinase (MAPK) pathways. FGF2 inhibited TGFβ-mediated fibroblast activation, resulting in more rapidly proliferating, spindle-shaped cells, compared to the more slowly proliferating, flatter TGFβ-treated cells. FGF2 is an important regulator of cell growth and differentiation under physiological and pathological conditions. FGF2 is widely involved in important biological processes such as stem cell proliferation and angiogenesis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Basic fibroblast growth factor, BFGF, FGF2, FGFB, HBGF 2 |
Publications
| Species | Sample Type | Title |
|---|---|---|
Food Res Int Efficient expression of a cytokine combination in Saccharomyces cerevisiae for cultured meat production | ||
Biotechnol J Evaluation of expression systems for recombinant protein production in chicken egg bioreactors | ||
J Dent Calvaria defect regeneration via human periodontal ligament stem cells and prevascularized scaffolds in athymic rats | ||
ACS Nano Controllably Self-Assembled Antibacterial Nanofibrils Based on Insect Cuticle Protein for Infectious Wound Healing | ||
BMC Cardiovasc Disord Serum fibroblast growth factor-2 levels complement vital biomarkers for diagnosing heart failure | ||
Chin Med Isoforskolin modulates AQP4-SPP1-PIK3C3 related pathway for chronic obstructive pulmonary disease via cAMP signaling |