Human FGFR3 ELISA Kit
Cat no : KE00401
Synonyms
FGFR3,ACH,CD333,CEK2,FGFR 3
Validation Data Gallery
Product Information
KE00401 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human FGFR3 ELISA kit is to be used to detect and quantify protein levels of endogenous human FGFR3. The assay recognizes human FGFR3. An antibody specific for human FGFR3 has been pre-coated onto the microwells. The human FGFR3 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human FGFR3 is added to detect the captured human FGFR3 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human FGFR3 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.04 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2261 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 98% | 86%-120% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 5.02 | 0.41 | 8.15 |
| 2 | 20 | 1.18 | 0.10 | 8.85 |
| 3 | 20 | 0.26 | 0.02 | 7.25 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 4.52 | 0.41 | 9.12 |
| 2 | 24 | 1.20 | 0.11 | 8.92 |
| 3 | 24 | 0.36 | 0.03 | 9.74 |
Background Information
Fibroblast growth factors (FGFs) are polypeptide growth factors involved in various activities including mitogenesis, angiogenesis, and wound healing. The human FGF receptor family, a subfamily of receptor tyrosine kinases (RTKs), comprises four family members-FGFR1, FGFR2, FGFR3, and FGFR4. FGFR3 (Fibroblast growth factor receptor 3), plays critical roles in cell cycle and angiogenesis processes and especially in cartilage development and homeostasis. As a physiological regulator in linear bone growth, FGFR3 negatively regulates chondrocyte proliferation and differentiation by activating downstream signaling pathways to maintain reconstructive balance in the growth plate.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | FGFR3,ACH,CD333,CEK2,FGFR 3 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.