Human FGR ELISA Kit
Cat no : KE00528
Synonyms
FGR,c fgr,c src2,p55 Fgr,p55c fgr
Validation Data Gallery
Product Information
KE00528 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human FGR ELISA kit is to be used to detect and quantify protein levels of endogenous human FGR. The assay recognizes human FGR. An antibody specific for human FGR has been pre-coated onto the microwells. The human FGR protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human FGR is added to detect the captured human FGR protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human FGR ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.05 ng/mL |
| Range | 0.78-50 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2268 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 87% | 78%-113% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 23.65 | 1.05 | 4.45 |
| 2 | 8 | 6.16 | 0.21 | 3.43 |
| 3 | 8 | 1.53 | 0.09 | 6.18 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 23.66 | 1.09 | 4.60 |
| 2 | 16 | 5.96 | 0.36 | 6.02 |
| 3 | 16 | 1.64 | 0.14 | 8.66 |
Background Information
FGR (Tyrosine-protein kinase Fgr), also known as SRC2. The protein is detected in neutrophils, monocytes and natural killer cells (at protein level)(PMID: 11078731). FGR protein plays an important role in cell migration, adhesion, cytoskeleton remodeling, phagocytosis and immune response. It negatively regulates cell migration and adhesion through β2 integrin signaling pathway. In addition, FGR is also involved in regulating the degranulation of basophils, the release of inflammatory cytokines and IgE-mediated allergic reactions. FGR is expressed in bone marrow, spleen and other tissues, especially in myeloid cells and tissue macrophages.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | FGR,c fgr,c src2,p55 Fgr,p55c fgr |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.