Human FKBP11 ELISA Kit
Cat no : KE00595
Synonyms
19 kDa FK506 binding protein, 19 kDa FK506-binding protein, 19 kDa FKBP, EC:5.2.1.8, FK506 binding protein 11
Validation Data Gallery
Product Information
KE00595 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human FKBP11 ELISA kit is to be used to detect and quantify protein levels of endogenous human FKBP11. The assay recognizes human FKBP11. An antibody specific for human FKBP11 has been pre-coated onto the microwells. The human FKBP11 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human FKBP11 is added to detect the captured human FKBP11 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human FKBP11 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.02 ng/mL |
| Range | 0.625-40 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 51303 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 88% | 86%-90% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 26.43 | 1.89 | 7.17 |
| 2 | 8 | 4.93 | 0.31 | 6.32 |
| 3 | 8 | 2.50 | 0.18 | 7.07 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 25.33 | 2.10 | 8.30 |
| 2 | 16 | 4.81 | 0.29 | 6.06 |
| 3 | 16 | 2.58 | 0.16 | 6.05 |
Background Information
FKBP11 (FK506 binding protein 11, also named FKBP19) was first described as predominantly expressed in secretory tissues, including the pancreas, and recent studies have suggested a role in beta cell survival under conditions of ER stress. In addition, FKBP11 appears to play a role in osteoblasts and bone formation, where it has been observed to associate with interferon-inducible transmembrane protein 5 (IFITM5).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | 19 kDa FK506 binding protein, 19 kDa FK506-binding protein, 19 kDa FKBP, EC:5.2.1.8, FK506 binding protein 11 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.