Human FLRG ELISA Kit
Cat no : KE00258
Synonyms
FLRG, Follistatin like protein 3, Follistatin related protein 3, FSRP, FSTL3
Validation Data Gallery
Product Information
KE00258 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human FLRG ELISA kit is to be used to detect and quantify protein levels of endogenous human FLRG. The assay recognizes human FLRG. An antibody specific for human FLRG has been pre-coated onto the microwells. The human FLRG protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human FLRG is added to detect the captured human FLRG protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human FLRG ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Urine, Human milk |
| Assay type | Sandwich |
| Sensitivity | 4.3 pg/mL |
| Range | 31.25-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 10272 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 96% | 80%-106% |
| Cell culture supernatants | 93% | 70%-109% |
| Urine | 93% | 72%-121% |
| Human milk | 90% | 70%-117% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 478.8 | 18.0 | 3.8 |
| 2 | 20 | 115.8 | 4.1 | 3.5 |
| 3 | 20 | 23.4 | 1.9 | 8.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 476.7 | 12.2 | 2.6 |
| 2 | 24 | 120.6 | 2.3 | 1.9 |
| 3 | 24 | 15.8 | 1.5 | 9.5 |
Background Information
Follistatin-like 3 (FSTL3), also called follistatin-related protein or follistatin-related gene (FLRG) protein, is a highly conserved monomer secreted glycoprotein located on chromosome 19p13.3. The FSTL3 gene is the target of a novel chromosomal rearrangement discovered by Hayette et al in 1998 in B cells of patients with chronic lymphocytic leukemia. FSTL3 can modulate target gene expression via members of the transforming growth factor β (TGF-β) superfamily. Recent literatures have revealed that FSTL3 regulates multiple biological processes, including cell differentiation, aging, obesity development, arteriosclerosis development, and tumor progression.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | FLRG, Follistatin like protein 3, Follistatin related protein 3, FSRP, FSTL3 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Cell Death Dis Cancer-associated fibroblasts expressing FSTL3 promote vasculogenic mimicry formation and drive colon cancer malignancy. |