Human FLT3 ELISA Kit
Cat no : KE00868
Synonyms
CD135, Fetal liver kinase-2, FL cytokine receptor, FLK2, FLK-2
Validation Data Gallery
Product Information
KE00868 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human FLT3 ELISA kit is to be used to detect and quantify protein levels of endogenous human FLT3. The assay recognizes human FLT3. An antibody specific for human FLT3 has been pre-coated onto the microwells. The human FLT3 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human FLT3 is added to detect the captured human FLT3 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human FLT3 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 6.4 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2322 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 97% | 80%-110% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,086.6 | 96.8 | 4.6 |
| 2 | 8 | 504.8 | 36.7 | 7.3 |
| 3 | 8 | 293.2 | 14.6 | 5.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,050.9 | 98.5 | 4.8 |
| 2 | 16 | 496.3 | 35.3 | 7.1 |
| 3 | 16 | 269.6 | 26.6 | 9.9 |
Background Information
FLT3 protein, also known as Fms-like tyrosine kinase 3, is a receptor tyrosine kinase involved in hematopoietic cell proliferation and differentiation. It is highly expressed in acute myeloid leukemia (AML) and plays a crucial role in disease progression. FLT3 mutations, particularly internal tandem duplications (ITDs) and tyrosine kinase domain mutations, are associated with poor prognosis in AML. Targeting FLT3 has become an important therapeutic strategy in AML treatment. Additionally, FLT3 ligand is essential for the maintenance and expansion of hematopoietic stem and progenitor cells.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD135, Fetal liver kinase-2, FL cytokine receptor, FLK2, FLK-2 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.