Human FOXC2 ELISA Kit
Cat no : KE00515
Synonyms
FKHL14, Forkhead box protein C2, Forkhead-related protein FKHL14, Mesenchyme fork head protein 1, MFH 1
Validation Data Gallery
Product Information
KE00515 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human FOXC2 ELISA kit is to be used to detect and quantify protein levels of endogenous human FOXC2. The assay recognizes human FOXC2. An antibody specific for human FOXC2 has been pre-coated onto the microwells. The human FOXC2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human FOXC2 is added to detect the captured human FOXC2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human FOXC2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 4.3 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2303 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 99% | 94%-114% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,852.9 | 80.0 | 2.8 |
| 2 | 8 | 647.9 | 14.6 | 2.2 |
| 3 | 8 | 198.0 | 9.7 | 4.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,746.1 | 233.3 | 8.5 |
| 2 | 16 | 648.5 | 19.6 | 3.0 |
| 3 | 16 | 197.1 | 8.0 | 4.1 |
Background Information
Forkhead box protein C2 (FOXC2) also known as forkhead-related protein FKHL14 (FKHL14), transcription factor FKH-14, or mesenchyme fork head protein 1 (MFH1) is a protein that in humans is encoded by the FOXC2 gene. FOXC2 is a member of the fork head box (FOX) family of transcription factors. FOX transcription factors are expressed during development and are associated with a number of cellular and developmental differentiation processes. FOXC2 is required during early development of the kidneys, including differentiation of podocytes and maturation of the glomerular basement membrane. It is also involved in the early development of the heart. FOXC2 is also involved in cancer metastases. In particular, expression of FOXC2 is induced when epithelial cells undergo an epithelial-mesenchymal transition (EMT) and become mesenchymal looking cells.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | FKHL14, Forkhead box protein C2, Forkhead-related protein FKHL14, Mesenchyme fork head protein 1, MFH 1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.