Human FOXM1 ELISA Kit
Cat no : KE00724
Synonyms
FKHL16, Forkhead-related protein FKHL16, HFH11, HFH-11, HNF-3/fork-head homolog 11
Validation Data Gallery
Product Information
KE00724 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human FOXM1 ELISA kit is to be used to detect and quantify protein levels of endogenous human FOXM1. The assay recognizes human FOXM1. An antibody specific for human FOXM1 has been pre-coated onto the microwells. The human FOXM1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human FOXM1 is added to detect the captured human FOXM1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human FOXM1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.02 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2305 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 95% | 92%-96% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 4.24 | 0.26 | 6.04 |
| 2 | 8 | 1.16 | 0.11 | 9.85 |
| 3 | 8 | 0.56 | 0.05 | 8.29 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 4.07 | 0.40 | 9.93 |
| 2 | 16 | 1.12 | 0.11 | 9.87 |
| 3 | 16 | 0.54 | 0.05 | 9.89 |
Background Information
FOXM1 (Forkhead Box M1) is a transcription factor that plays crucial roles in cell cycle progression, proliferation, and cancer development. It is highly expressed in various cancers, including breast cancer, lung cancer, and gastric cancer, and is associated with poor prognosis. FOXM1 promotes tumor growth and metastasis by regulating genes involved in cell cycle progression and epithelial-mesenchymal transition. It is also involved in chemoresistance and can be regulated by various signaling pathways, such as Wnt/β-catenin and TGF-β. Additionally, FOXM1 is a potential target for cancer therapy due to its critical role in tumor progression.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | FKHL16, Forkhead-related protein FKHL16, HFH11, HFH-11, HNF-3/fork-head homolog 11 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.