Human FOXO1 ELISA Kit
Cat no : KE00622
Synonyms
FKH 1, FKH1, FKHR, forkhead box O1, Forkhead box protein O1
Validation Data Gallery
Product Information
KE00622 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human FOXO1 ELISA kit is to be used to detect and quantify protein levels of endogenous human FOXO1. The assay recognizes human FOXO1. An antibody specific for human FOXO1 has been pre-coated onto the microwells. The human FOXO1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human FOXO1 is added to detect the captured human FOXO1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human FOXO1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 9.7 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2308 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 107% | 100%-120% |
| Cell lysate | 94% | 84%-102% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,114.9 | 100.2 | 9.0 |
| 2 | 8 | 238.6 | 21.3 | 8.9 |
| 3 | 8 | 128.0 | 10.1 | 7.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,103.9 | 106.6 | 9.7 |
| 2 | 16 | 262.4 | 26.3 | 10.0 |
| 3 | 16 | 133.2 | 14.1 | 10.6 |
Background Information
The FOXO, a subgroup of FOX proteins, is composed of four members including FOXO1, FOXO3, FOXO4, and FOXO6, which control particular genes to govern cell-cycle arrest, apoptosis, DNA damage repair, and cell growth. FOXO1, the most widely studied subtype, has attracted much more attention due to its important role in lung cancer. Except for growth factors, various signaling pathways and upstream protein regulators such as PI3K/AKT, SIRTs, reactive oxygen species (ROS), and non-coding RNAs (ncRNAs) may positively or negatively modulate FOXO1 function.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | FKH 1, FKH1, FKHR, forkhead box O1, Forkhead box protein O1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.