Human FSTL1 ELISA Kit
Cat no : KE00744
Synonyms
follistatin like 1, Follistatin like protein 1, Follistatin related protein 1, Follistatin-like protein 1, Follistatin-related protein 1
Validation Data Gallery
Product Information
KE00744 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human FSTL1 ELISA kit is to be used to detect and quantify protein levels of endogenous human FSTL1. The assay recognizes human FSTL1. An antibody specific for human FSTL1 has been pre-coated onto the microwells. The human FSTL1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human FSTL1 is added to detect the captured human FSTL1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human FSTL1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 0.18 ng/mL |
| Range | 0.625-40 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 11167 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Lysate | 80% | 71%-93% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 14.50 | 0.76 | 5.24 |
| 2 | 8 | 4.25 | 0.24 | 5.68 |
| 3 | 8 | 2.48 | 0.24 | 9.73 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 15.35 | 1.46 | 9.49 |
| 2 | 16 | 4.15 | 0.27 | 6.57 |
| 3 | 16 | 2.38 | 0.21 | 8.90 |
Background Information
Follistatin-like 1 (Fstl1) is a member of the secreted protein acidic rich in cysteins (SPARC) family and has been implicated in many different signaling pathways, including bone morphogenetic protein (BMP) signaling. In many different developmental processes like, dorso-ventral axis establishment, skeletal, lung and ureter development, loss of function experiments have unveiled an important role for Fstl1. Fstl1 largely functions through inhibiting interactions with the BMP signaling pathway, although, in various disease models, different signaling pathways, like activation of pAKT, pAMPK, Na/K-ATPase, or innate immune responses, are linked to Fstl1. It has been proposed that Fstl1 interferes with BMP receptor complex formation and as such inhibits propagation of the BMP signal into the cell.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | follistatin like 1, Follistatin like protein 1, Follistatin related protein 1, Follistatin-like protein 1, Follistatin-related protein 1 |