Human G-CSFR ELISA Kit
Cat no : KE00723
Synonyms
CD114, CSF3R, G CSF R, G CSF receptor, G-CSF receptor
Validation Data Gallery
Product Information
KE00723 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human G-CSFR ELISA kit is to be used to detect and quantify protein levels of endogenous human G-CSFR. The assay recognizes human G-CSFR. An antibody specific for human G-CSFR has been pre-coated onto the microwells. The human G-CSFR protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human G-CSFR is added to detect the captured human G-CSFR protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human G-CSFR ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.06 ng/mL |
| Range | 0.2-12.5 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1441 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 84% | 80%-89% |
| Cell lysate | 85% | 77%-95% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 5.77 | 0.36 | 6.17 |
| 2 | 8 | 1.37 | 0.12 | 8.47 |
| 3 | 8 | 0.75 | 0.07 | 9.31 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 5.86 | 0.44 | 7.51 |
| 2 | 16 | 1.34 | 0.10 | 7.64 |
| 3 | 16 | 0.72 | 0.07 | 10.00 |
Background Information
Granulocyte colony-stimulating factor receptor (G-CSFR) is a receptor for granulocyte colony-stimulating factor (G-CSF) and plays a crucial role in regulating neutrophil development and function. G-CSFR is essential for the proliferation, differentiation, and survival of neutrophil lineage cells. It mediates the specific effects of G-CSF through activating various intracellular signaling pathways, including the Jak/Stat, PI3/Akt, Ras-Raf-MAP kinase, and Src family kinase pathways. These pathways are involved in defense against infection, inflammation, and repair, as well as in maintaining steady-state hematopoiesis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD114, CSF3R, G CSF R, G CSF receptor, G-CSF receptor |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.