Human G3BP2 ELISA Kit
Cat no : KE00429
Synonyms
G3BP 2,G3BP2,G3BP-2,GAP SH3 domain binding protein
Validation Data Gallery
Product Information
KE00429 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human G3BP2 ELISA kit is to be used to detect and quantify protein levels of endogenous human G3BP2. The assay recognizes human G3BP2. An antibody specific for human G3BP2 has been pre-coated onto the microwells. The human G3BP2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human G3BP2 is added to detect the captured human G3BP2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human G3BP2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.52 ng/mL |
| Range | 0.313 - 20 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 9908 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 87% | 81%-93% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 7.44 | 0.40 | 5.37 |
| 2 | 20 | 1.64 | 0.06 | 3.85 |
| 3 | 20 | 0.39 | 0.03 | 8.71 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 7.44 | 0.37 | 5.00 |
| 2 | 24 | 1.54 | 0.11 | 6.85 |
| 3 | 24 | 0.35 | 0.03 | 8.77 |
Background Information
Stress granules (SGs) are cytoplasmic mRNA-protein condensates formed in response to cellular stressors, such as oxidative stress, ultraviolet radiation, and viral infection. The Ras-GTPase-activating protein-binding proteins (G3BPs), consisting of G3BP1 and G3BP2, are key nucleating factors essential for SG formation. They function to protect RNAs from harmful conditions. G3BP2 is mainly distributed in the cytoplasm and participates in the formation of stress granules, cell differentiation, proliferation, and signal transduction. Accumulating evidence has demonstrated that aberrant expression of G3BP2 contributes to cancer initiation and progression, such as high expression of G3BP2 increasing cell stemness, metastasis and chemoresistance in breast cancer.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | G3BP 2,G3BP2,G3BP-2,GAP SH3 domain binding protein |