Human GABPA ELISA Kit
Cat no : KE00704
Synonyms
E4TF1 60, E4TF1A, GA binding protein alpha chain, GA-binding protein alpha chain, GABP subunit alpha
Validation Data Gallery
Product Information
KE00704 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human GABPA ELISA kit is to be used to detect and quantify protein levels of endogenous human GABPA. The assay recognizes human GABPA. An antibody specific for human GABPA has been pre-coated onto the microwells. The human GABPA protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human GABPA is added to detect the captured human GABPA protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human GABPA ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 5.1 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2551 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 95% | 87%-100% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,327.0 | 227.5 | 9.8 |
| 2 | 8 | 585.6 | 49.3 | 8.4 |
| 3 | 8 | 254.6 | 25.2 | 9.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,302.6 | 188.5 | 8.2 |
| 2 | 16 | 562.1 | 53.7 | 9.6 |
| 3 | 16 | 265.1 | 25.6 | 9.6 |
Background Information
GA-binding protein alpha chain (GABP alpha subunit, GABPA, nuclear respiratory factor 2 subunit alpha, transcription factor E4TF1-60) is one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit. GABPA is a member of Ets family, binds to the Yap promoter and activates YAP transcription. Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. GABPA plays an important role in regulating genes involved in various biological processes such as embryonic development, cell differentiation, cell cycle, and mitochondrial biogenesis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | E4TF1 60, E4TF1A, GA binding protein alpha chain, GA-binding protein alpha chain, GABP subunit alpha |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.