Human GBE1 ELISA Kit
Cat no : KE00962
Synonyms
1,4-alpha-glucan-branching enzyme, APBD, EC:2.4.1.18, GBE, Glycogen-branching enzyme
Validation Data Gallery
Product Information
KE00962 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human GBE1 ELISA kit is to be used to detect and quantify protein levels of endogenous human GBE1. The assay recognizes human GBE1. An antibody specific for human GBE1 has been pre-coated onto the microwells. The human GBE1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human GBE1 is added to detect the captured human GBE1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human GBE1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.54 ng/mL |
| Range | 1.56-100 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2632 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 91% | 86%-95% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 53.33 | 2.80 | 5.25 |
| 2 | 8 | 12.11 | 0.39 | 3.19 |
| 3 | 8 | 5.65 | 0.28 | 4.91 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 50.19 | 4.67 | 9.30 |
| 2 | 16 | 11.68 | 1.08 | 9.25 |
| 3 | 16 | 5.38 | 0.48 | 8.98 |
Background Information
Glucan Branching Enzyme 1 (GBE1), mainly distributed in liver and muscle tissue, is a glycogen branching enzyme and involved in the transportation of glucosyl to glycogen chain, which is essential for glycogen accumulation and its solubility upregulation. Mutations in the GBE1 gene can lead to glycogen storage disease type IV, a severe metabolic disorder. This enzyme is also implicated in cancer progression, as studies have shown that GBE1 can promote tumor growth through metabolic reprogramming. Additionally, GBE1 has been identified as a potential therapeutic target in glioma due to its role in aerobic glycolysis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | 1,4-alpha-glucan-branching enzyme, APBD, EC:2.4.1.18, GBE, Glycogen-branching enzyme |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.