Human GBP1 ELISA Kit
Cat no : KE00420
Synonyms
GBP1,EC:3.6.1.-,GBP 1,GBP-1,GTP-binding protein 1
Validation Data Gallery
Product Information
KE00420 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human GBP1 ELISA kit is to be used to detect and quantify protein levels of endogenous human GBP1. The assay recognizes human GBP1. An antibody specific for human GBP1 has been pre-coated onto the microwells. The human GBP1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human GBP1 is added to detect the captured human GBP1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human GBP1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 1.3 pg/mL |
| Range | 54.7-3500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2633 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 100% | 95%-108% |
| Cell culture supernatant | 101% | 92%-106% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1,806.9 | 117.9 | 6.5 |
| 2 | 20 | 443.7 | 22.1 | 5.0 |
| 3 | 20 | 131.7 | 8.6 | 6.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,745.4 | 133.8 | 7.7 |
| 2 | 24 | 423.1 | 26.1 | 6.2 |
| 3 | 24 | 134.6 | 10.1 | 7.5 |
Background Information
GBP1 is the most concerned member of the GBP family, originally extracted from interferon (IFN) induced human fibroblasts, and has the unique features of binding with guanosine triphosphate (GTP), guanosine diphosphate (GDP) and guanosine monophosphate (GMP), hence the name. GBP1 plays a crucial role in innate immunity, comprising a conserved region with an N-terminal globular GTPase domain and a C-terminal helical domain. It can mediate host defense and has the function of resisting various viruses, bacteria, and protozoa pathogens. It also can mediate inflammatory cytokines to inhibit the proliferation, diffusion, and migration of endothelial cells, thus playing an anti-angiogenesis role. GBP1 is a protein found in many mammals and most vertebrates, and it is also commonly expressed in various types of tumors, including colorectal and prostatic cancers.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | GBP1,EC:3.6.1.-,GBP 1,GBP-1,GTP-binding protein 1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.