Human GCSH ELISA Kit
Cat no : KE00532
Synonyms
GCSH, GCE, Glycine cleavage system H protein, mitochondrial, MMDS7, NKH
Validation Data Gallery
Product Information
KE00532 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human GCSH ELISA kit is to be used to detect and quantify protein levels of endogenous human GCSH. The assay recognizes human GCSH. An antibody specific for human GCSH has been pre-coated onto the microwells. The human GCSH protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human GCSH is added to detect the captured human GCSH protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human GCSH ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 16.8 pg/mL |
| Range | 39-2500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2653 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 100% | 90%-121% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,151.8 | 92.3 | 8.0 |
| 2 | 8 | 283.5 | 17.3 | 6.1 |
| 3 | 8 | 154.2 | 5.7 | 3.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,172.9 | 69.9 | 6.0 |
| 2 | 16 | 294.7 | 18.9 | 6.4 |
| 3 | 16 | 147.4 | 12.6 | 8.6 |
Background Information
GCSH (glycine cleavage system H protein, mitochondrial) is a component of the glycine cleavage system loosely associated with the inner mitochondrial membrane and has lipoic acid as a prosthetic group. The full-length GCSH cDNA encodes a precursor protein of 173 amino acids and a mature protein of 125 amino acids. Lipoylation of the H-protein occurs in mitochondria, which are thought to contain an activated form of lipoic acid as well as other components necessary for the transfer of lipoic acid to the protein. Defects in GCSH are a cause of non-ketotic hyperglycaemia (NKH).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | GCSH, GCE, Glycine cleavage system H protein, mitochondrial, MMDS7, NKH |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.