Human GDF-15 ELISA Kit

Sensitivity

0.3 ng/mL

Range

1.25-40 ng/mL

Reactivity

Human

Cat no : KE00108

Synonyms

GDF 15, GDF15, MIC 1, MIC1, NAG 1, NRG 1, NSAID activated gene 1 protein, NSAID regulated gene 1 protein, PDF, PLAB, Placental TGF beta, PTGFB



Product Information

KE00108 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The GDF-15 ELISA kit is to be used to detect and quantify protein levels of endogenous GDF-15. The assay recognizes human GDF-15. An antibody specific for GDF-15 has been pre-coated onto the microwells. The GDF-15 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for GDF-15 is added to detect the captured GDF-15 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Human GDF-15 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants, Urine
Assay type Sandwich
Sensitivity 0.3 ng/mL
Range 1.25-40 ng/mL
Reactivity Human
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
Cell culture supernatants107%93%-119%
Urine86%73%-99%
Human plasma79%71%-92%
Human serum83%73%-91%

IntraAssay

Samplenmean ( ng/mL)SDCV%
1206.40.69.4
22015.11.610.4
32040.62.97.1

InterAssay

Samplenmean ( ng/mL)SDCV%
1246.80.45.1
22413.90.96.3
32442.51.43.3

Background Information

Growth differentiation factor 15 (GDF-15), also known as macrophage inhibitory cytokine-1 (MIC-1), is a protein of the transforming growth factor beta (TGFb) superfamily. GDF-15 is a stress-induced cytokine and associated with hypoxia, inflammation and oxidative stress, it is also released from endothelial cells after stimulation with pro-inflammatory cytokines. Its levels are elevated in diseases such as acute respiratory distress syndrome, pulmonary hypertension and heart failure. GDF-15 is associated with cancers, cardiovascular diseases, and inflammatory diseases. GDF-15 may have a paracrine role in the modulation of adipose tissue function and body fat mass. GDF-15 is highly expressed in the placenta. GDF-15 is known to be involved in human embryo development and necessary for the maintenance of pregnancy.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms GDF 15, GDF15, MIC 1, MIC1, NAG 1, NRG 1, NSAID activated gene 1 protein, NSAID regulated gene 1 protein, PDF, PLAB, Placental TGF beta, PTGFB