Human GLUD2 ELISA Kit
Cat no : KE00984
Synonyms
EC:1.4.1.3, GDH 2, GDH2, GLUD 2, GLUDP1
Validation Data Gallery
Product Information
KE00984 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human GLUD2 ELISA kit is to be used to detect and quantify protein levels of endogenous human GLUD2. The assay recognizes human GLUD2. An antibody specific for human GLUD2 has been pre-coated onto the microwells. The human GLUD2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human GLUD2 is added to detect the captured human GLUD2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human GLUD2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.01 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2747 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 95% | 84%-114% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 5.02 | 0.09 | 1.88 |
| 2 | 8 | 1.33 | 0.06 | 4.56 |
| 3 | 8 | 0.61 | 0.04 | 7.29 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 5.43 | 0.52 | 9.60 |
| 2 | 16 | 1.37 | 0.07 | 5.10 |
| 3 | 16 | 0.60 | 0.06 | 10.35 |
Background Information
Glutamate dehydrogenase 2 (GLUD2) catalyzes the reversible interconversion of glutamate to α-KG and ammonia while reducing NAD(P) + to NAD(P)H as cofactors. This enzyme plays a key role in cellular homeostasis, being at the interface between amino acid and carbohydrate metabolism. GLUD2 is linked to important cellular processes including Krebs cycle, ammonia control and energy generation.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | EC:1.4.1.3, GDH 2, GDH2, GLUD 2, GLUDP1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.