Human GMFG ELISA Kit
Cat no : KE00087
Synonyms
Glia maturation factor gamma, glia maturation factor, gamma, GMF GAMMA, GMFG
Validation Data Gallery
Product Information
KE00087 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The GMFG ELISA kit is to be used to detect and quantify protein levels of endogenous GMFG. The assay recognizes human GMFG. An antibody specific for GMFG has been pre-coated onto the microwells. The GMFG protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for GMFG is added to detect the captured GMFG protein. For signal development,horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent.Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human GMFG ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 13.0 pg/mL |
| Range | 39-2500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 9535 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 96% | 76%-128% |
| Cell culture supernatants | 90% | 78%-104% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 49.6 | 2.2 | 4.4 |
| 2 | 20 | 461.8 | 8.4 | 1.8 |
| 3 | 20 | 1,844.2 | 70.9 | 3.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 45.6 | 2.6 | 5.7 |
| 2 | 24 | 464.3 | 17.1 | 3.7 |
| 3 | 24 | 1,851.2 | 29.0 | 1.6 |
Background Information
GMFG (glia maturation factor-γ), is a highly conserved brain-specific protein that belongs to the GMF subfamily of the larger Actin-binding protein ADF family. GMFG is preferentially expressed in blood vessel cells and immune cells and its ectopic expression enhances cellular functions such as tube-formation and migration in vitro. GMFG mediates neutrophil and T-lymphocyte migration via regulation of actin cytoskeletal reorganization. GMFG also acts as an intracellular regulator of stress-activated signal transduction by demonstrating activation of p38 MAP kinase and transcription factor NF-kB in astrocytes.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Glia maturation factor gamma, glia maturation factor, gamma, GMF GAMMA, GMFG |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.