Human GNLY/Granulysin ELISA Kit
Cat no : KE00200
Synonyms
519, D2S69E, GNLY, granulysin, LAG 2, LAG2, Lymphokine LAG 2, NKG5, Protein NKG5, T cell activation protein 519, TLA519
Validation Data Gallery
Product Information
KE00200 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The GNLY/Granulysin ELISA kit is to be used to detect and quantify protein levels of endogenous GNLY/Granulysin. The assay recognizes human GNLY/Granulysin. An antibody specific for GNLY/Granulysin has been pre-coated onto the microwells. The GNLY/Granulysin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for GNLY/Granulysin is added to detect the captured GNLY/Granulysin protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human GNLY/Granulysin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 0.6 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 10578 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 97% | 80%-111% |
| Cell culture supernatants | 82% | 75%-98% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 259.2 | 9.5 | 3.7 |
| 2 | 20 | 66.1 | 1.9 | 2.9 |
| 3 | 20 | 14.8 | 0.5 | 3.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 262.6 | 13.7 | 5.2 |
| 2 | 24 | 60.8 | 3.9 | 6.4 |
| 3 | 24 | 13.2 | 0.7 | 5.5 |
Background Information
Granulysin (GNLY) is a member of the saposin-like protein (SAPLIP) family that is a cytolytic and proinflammatory molecule expressed by activated human cytotoxic T cells and NK cells, exhibiting lytic activity against a variety of microorganisms and tumors. Granulysin is produced as an intact 15-kDa form, and portions are then cleaved at the amino and carboxy termini to produce a 9-kDa form. Granulysin has broad clinical relevance to a wide variety of diseases, including infections, cancer, transplantation, skin afflictions and reproductive complications. Elevation of Granulysin expression and levels in tissue and serum has been reported in infections, autoimmune diseases, transplant rejection. Granulysin expression has been widely correlated with good outcomes in a variety of cancers.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | 519, D2S69E, GNLY, granulysin, LAG 2, LAG2, Lymphokine LAG 2, NKG5, Protein NKG5, T cell activation protein 519, TLA519 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.