Human GP2 ELISA Kit
Cat no : KE00876
Synonyms
Pancreatic zymogen granule membrane protein GP-2, ZAP75
Validation Data Gallery
Product Information
KE00876 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human GP2 ELISA kit is to be used to detect and quantify protein levels of endogenous human GP2. The assay recognizes human GP2. An antibody specific for human GP2 has been pre-coated onto the microwells. The human GP2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human GP2 is added to detect the captured human GP2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human GP2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 7.7 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2813 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 81% | 79%-82% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,468.0 | 121.6 | 4.9 |
| 2 | 8 | 770.3 | 16.0 | 2.1 |
| 3 | 8 | 248.8 | 6.1 | 2.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,483.7 | 114.1 | 4.6 |
| 2 | 16 | 756.3 | 19.2 | 2.5 |
| 3 | 16 | 251.3 | 13.0 | 5.2 |
Background Information
Glycoprotein 2 (GP2) is the most abundant membrane component of zymogen granules secreted by pancreatic acinar cells. GP2 associates with the plasma membrane via glycosylphosphatidylinositol (GPI) linkage. It is released from the membrane of mature zymogen granules by an enzymatic mechanism. GP2 plays a role in defense against adhesive and invasive commensal bacteria during intestinal inflammation. GP2 is preferentially expressed in acinar cell carcinomas of the pancreas, but the glycoprotein can also be expressed in a variety of other tumor entities.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Pancreatic zymogen granule membrane protein GP-2, ZAP75 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.