Human GPAM ELISA Kit
Cat no : KE00561
Synonyms
GPAM, EC:2.3.1.15, Glycerol-3-phosphate acyltransferase 1, mitochondrial, GPAT 1, GPAT1
Validation Data Gallery
Product Information
KE00561 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human GPAM ELISA kit is to be used to detect and quantify protein levels of endogenous human GPAM. The assay recognizes human GPAM. An antibody specific for human GPAM has been pre-coated onto the microwells. The human GPAM protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human GPAM is added to detect the captured human GPAM protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human GPAM ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.90 ng/mL |
| Range | 1.95-125 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 57678 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 97% | 79%-117% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 63.51 | 5.00 | 7.87 |
| 2 | 8 | 10.15 | 0.77 | 7.61 |
| 3 | 8 | 6.62 | 0.42 | 6.36 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 63.84 | 5.36 | 8.39 |
| 2 | 16 | 15.44 | 0.91 | 5.89 |
| 3 | 16 | 8.21 | 0.48 | 5.88 |
Background Information
GPAM (Glycerol-3-phosphate acyltransferase 1, mitochondrial) is an enzyme that plays a crucial role in lipid metabolism, specifically in the biosynthesis of glycerophospholipids(PMID: 28131819; 16276094). This metabolic pathway's first step is catalyzed by the encoded enzyme. Two forms for this enzyme exist, one in the mitochondria and one in the endoplasmic reticulum. Two alternatively spliced transcript variants have been described for this gene.Diseases associated with GPAM include Rhizomelic Chondrodysplasia Punctata, Type 2 and Congenital Generalized Lipodystrophy.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | GPAM, EC:2.3.1.15, Glycerol-3-phosphate acyltransferase 1, mitochondrial, GPAT 1, GPAT1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.