Human GPNMB ELISA Kit
Cat no : KE00571
Synonyms
Hematopoietic growth factor inducible neurokinin-1 type, NMB, Osteoactivin, Transmembrane glycoprotein NMB, UNQ1725/PRO9925
Validation Data Gallery
Product Information
KE00571 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human GPNMB ELISA kit is to be used to detect and quantify protein levels of endogenous human GPNMB. The assay recognizes human GPNMB. An antibody specific for human GPNMB has been pre-coated onto the microwells. The human GPNMB protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human GPNMB is added to detect the captured human GPNMB protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human GPNMB ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Human serum |
| Assay type | Sandwich |
| Sensitivity | 0.28 ng/mL |
| Range | 0.78-50 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 10457 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 84% | 83%-84% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 25.32 | 0.47 | 1.86 |
| 2 | 8 | 6.39 | 0.25 | 3.85 |
| 3 | 8 | 3.15 | 0.15 | 4.70 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 25.33 | 0.59 | 2.33 |
| 2 | 16 | 6.44 | 0.26 | 4.00 |
| 3 | 16 | 3.16 | 0.15 | 4.76 |
Background Information
GPNMB (Glycoprotein non-metastatic melanoma protein B), also known as osteoactivin, is a type I transmembrane glycoprotein that plays a role in various physiological processes including cell differentiation, migration, inflammation, metabolism, and neuroprotection. It is expressed in multiple tissues such as bone, kidney, and skeletal muscle, and is implicated in cell processes like tissue regeneration and tumor progression. GPNMB has also been identified within motor neurons and radial glia, most abundantly in microglia cells, suggesting its role in inflammatory processes in the central nervous system. Its role in inflammation and tissue regeneration makes it a potential therapeutic target in diseases like atherosclerosis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Hematopoietic growth factor inducible neurokinin-1 type, NMB, Osteoactivin, Transmembrane glycoprotein NMB, UNQ1725/PRO9925 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.