Human GREB1 ELISA Kit
Cat no : KE00845
Synonyms
GREB1 protein, KIAA0575, Protein GREB1
Validation Data Gallery
Product Information
KE00845 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human GREB1 ELISA kit is to be used to detect and quantify protein levels of endogenous human GREB1. The assay recognizes human GREB1. An antibody specific for human GREB1 has been pre-coated onto the microwells. The human GREB1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human GREB1 is added to detect the captured human GREB1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human GREB1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.07 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 9687 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 102% | 93%-109% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 5.10 | 0.19 | 3.64 |
| 2 | 8 | 1.38 | 0.04 | 3.02 |
| 3 | 8 | 0.43 | 0.01 | 3.32 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 5.09 | 0.18 | 3.53 |
| 2 | 16 | 1.38 | 0.04 | 3.05 |
| 3 | 16 | 0.43 | 0.01 | 3.00 |
Background Information
GREB1 (Gene regulated by estrogen in breast cancer protein1) encodes a 1949-amino acid protein that contains an N-terminal Zn-binding domain followed by a circularly permuted catalytically inactive superfamily II helicase domain and a C-terminal Fringe-like GT domain. GREB1 is an early estrogen-responsive gene, and its expression is correlated with estrogen levels in breast cancer patients. GREB1 is implicated in hormone-dependent cancers, such as ovarian, prostate cancer and as a risk factor in endometriosis and variations in bone mineral density.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | GREB1 protein, KIAA0575, Protein GREB1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.