Human GRP78/BIP ELISA Kit
Cat no : KE00333
Synonyms
BIP, GRP78, HSPA5, 78 kDa glucose-regulated protein, Binding-immunoglobulin protein
Validation Data Gallery
Product Information
KE00333 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human GRP78/BIP ELISA kit is to be used to detect and quantify protein levels of endogenous human GRP78/BIP . The assay recognizes human GRP78/BIP. An antibody specific for human GRP78/BIP has been pre-coated onto the microwells. The human GRP78/BIP protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for human GRP78/BIP is added to detect the captured human GRP78/BIP protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human GRP78/BIP ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum,Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.02 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3309 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 89% | 71%-98% |
| Cell lysate | 99% | 85%-116% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 4.97 | 0.23 | 4.62 |
| 2 | 20 | 1.22 | 0.02 | 1.80 |
| 3 | 20 | 0.30 | 0.01 | 3.17 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 4.47 | 0.28 | 6.30 |
| 2 | 24 | 1.15 | 0.06 | 5.47 |
| 3 | 24 | 0.30 | 0.01 | 4.74 |
Background Information
GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER) (PMID: 2294010; PMID: 23769672). It is a constitutively expressed resident protein of the ER in all eukaryotic cells (PMID: 23990668). It has been reported that GRP78 is associated with apoptosis or inhibition of cancer cell growth (PMID: 26045166).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | BIP, GRP78, HSPA5, 78 kDa glucose-regulated protein, Binding-immunoglobulin protein |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Pathogens Zinc Acetate Inhibits Hepatitis A Virus Replication: Possible Treatment for Patients with Type A Acute-on-Chronic Liver Failure. |