Human/Cynomolgus Monkey Galectin 3 ELISA Kit
Sensitivity
0.01 ng/mL
Range
0.156-10 ng/mL
Reactivity
Human/Cynomolgus Monkey
Cat no : KE00311
Synonyms
Galectin-3, 35 kDa lectin, Carbohydrate-binding protein 35, CBP 35, CBP35
Validation Data Gallery
Product Information
KE00311 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human/cynomolgus monkey Galectin 3 ELISA kit is to be used to detect and quantify protein levels of endogenous human/cynomolgus monkey Galectin 3. The assay recognizes human/cynomolgus monkey Galectin 3. An antibody specific for human/cynomolgus monkey Galectin 3 has been pre-coated onto the microwells. The human/cynomolgus monkey Galectin 3 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human/cynomolgus monkey Galectin 3 is added to detect the captured human/cynomolgus monkey Galectin 3 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human/Cynomolgus Monkey Galectin 3 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Human serum, Human plasma, Cynomolgus monkey serum, Cynomolgus monkey plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 0.01 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human/Cynomolgus Monkey |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3958 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 96% | 85%-106% |
| Cynomolgus monkey serum | 97% | 88%-102% |
| Human cell culture supernatant | 106% | 93%-121% |
| Cynomolgus monkey cell culture supernatant | 97% | 92%-100% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 3.91 | 0.07 | 1.87 |
| 2 | 20 | 0.90 | 0.02 | 2.22 |
| 3 | 20 | 0.23 | 0.01 | 3.61 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 3.73 | 0.13 | 3.38 |
| 2 | 24 | 0.88 | 0.03 | 2.98 |
| 3 | 24 | 0.23 | 0.01 | 4.33 |
Background Information
Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides. Galectin-3, a 31-kDa member of the β-galactoside-binding proteins, contains one carbohydrate recognition domain (CRD) and a proline- and glycine-rich N-terminal domain through which is able to form oligomers. Galectin-3 is widely expressed in many normal tissues and a variety of tumors. It is found intracellularly in nucleus and cytoplasm or secreted outside of cell, being present on the cell surface or in the extracellular space. Galectin-3 is involved in various biological processes including cell growth, adhesion, differentiation, apoptosis, angiogenesis, immune response, neoplastic transformation and metastasis. Elevated serum galectin-3 levels have been reported in patients with breast, gastrointestinal, lung, or ovarian cancer, melanoma, and non-Hodgkin's lymphoma.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Galectin-3, 35 kDa lectin, Carbohydrate-binding protein 35, CBP 35, CBP35 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Int J Mol Sci Role of Endogenous Galectin-3 on Cell Biology of Immortalized Retinal Pigment Epithelial Cells In Vitro. | ||
Cell Commun Signal Galectin-3 in endometrial small extracellular vesicles promotes cytotrophoblast cell fusion during the implantation phase. | ||
Sci Rep Plasma levels of soluble PD-1, TIM-3, LAG-3 and galectin-3 and the degree of liver fibrosis in CHC and the impact of successful antiviral treatment on their levels |