Human Granzyme B ELISA Kit
Cat no : KE00121
Synonyms
GZMB, Cathepsin G-like 1, CCPI, CGL 1, CGL1
Validation Data Gallery
Product Information
KE00121 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Granzyme B ELISA kit is to be used to detect and quantify protein levels of endogenous Granzyme B. The assay recognizes human Granzyme B. An antibody specific for Granzyme B has been pre-coated onto the microwells. The Granzyme B protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for Granzyme B is added to detect the captured Granzyme B protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Granzyme B ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 2.6 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3002 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 85% | 77%-95% |
| Cell culture supernatants | 91% | 77%-99% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 26.6 | 1.0 | 3.8 |
| 2 | 20 | 112.0 | 5.2 | 4.6 |
| 3 | 20 | 465.7 | 31.7 | 6.8 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 27.4 | 2.7 | 9.7 |
| 2 | 24 | 119.4 | 7.2 | 6.0 |
| 3 | 24 | 499.8 | 27.7 | 5.5 |
Background Information
Granzyme B (GZMB) is also named as CGL1, CSPB, CTLA1, GRB and belongs to the Granzyme subfamily. This enzyme is necessary for target cell lysis in cell-mediated immune responses. The cytotoxic lymphocyte protease Granzyme B (GZMB) can promote apoptosis through direct processing and activation of members of the caspase family. Granzyme B also functions as processing protease of cytokines, including interleukin (IL)-18 and IL-1a, and extracellular matrix proteins, including fibronectin and various proteoglycans. Plasma Granzyme B concentrations were significantly higher in patients with Atopic dermatitis (AD) and psoriasis than in healthy controls, which indicated that Granzyme B may play an important role in the induction of intractable itch and dermatitis in AD.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | GZMB, Cathepsin G-like 1, CCPI, CGL 1, CGL1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Life Sci IFNβ-induced exosomal linc-EPHA6-1 promotes cytotoxicity of NK cells by acting as a ceRNA for hsa-miR-4485-5p to up-regulate NKp46 expression. | ||
Nanoscale Surface specifically modified NK-92 cells with CD56 antibody conjugated superparamagnetic Fe(3)O(4) nanoparticles for magnetic targeting immunotherapy of solid tumors | ||
Front Immunol Development and evaluation of an inhalable nanoemulsion system for enhancing NK cell function against osteosarcoma pulmonary metastases. | ||
Adv Sci (Weinh) CD20(+)FCRL4(+) B Cells Activate CD8(+) T Cells via MHC-I Restriction in Nasopharyngeal Carcinoma Anti-Tumor Immunity. |