Human HDAC1 ELISA Kit
Cat no : KE00033
Synonyms
EC:3.5.1.-, EC:3.5.1.98, GON 10, HD1, histone deacetylase 1
Validation Data Gallery
Product Information
KE00033 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The HDAC1 ELISA kit is to be used to detect and quantify protein levels of endogenous HDAC1. The assay recognizes human HDAC1. An antibody specific for HDAC1 has been pre-coated onto the microwells. The HDAC1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for HDAC1 is added to detect the captured HDAC1 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human HDAC1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 11.0 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3065 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 100% | 86%-127% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 2,406.1 | 197.3 | 8.2 |
| 2 | 20 | 499.5 | 19.9 | 4.0 |
| 3 | 20 | 163.5 | 6.3 | 3.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 2,282.9 | 158.9 | 7.0 |
| 2 | 24 | 484.8 | 24.5 | 5.1 |
| 3 | 24 | 157.3 | 6.9 | 4.4 |
Background Information
Histone deacetylases (HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. The protein encoded by this gene belongs to the histone deacetylase/ acuc/ apha family and is a component of the histone deacetylase complex, which is responsible for gene expression silencing. It also plays an important role in the control of cell proliferation and differentiation by interacting with RB, p53 and other transcription factors. HADC inhibition causes apoptosis in tumor cells and HDAC inhibitors may be developed as anti-cancer agents. This kit is used to quantify HDAC1 level in vivo.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | EC:3.5.1.-, EC:3.5.1.98, GON 10, HD1, histone deacetylase 1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.